Constitution of Melanin

Melanin, melanin "ghosts," and melanin composition in Cryptococcus neoformans.

Melanin synthesis is associated with virulence for the pathogenic fungus Cryptococcus neoformans. Exposure of nonmelanized C. neoformans 4067 cells to 4 M guanidinium isothiocyanate followed by 6 M HCl at 100 degrees C resulted in complete solubilization of cellular structures. However, exposure of melanized C. neoformans 24067 to the same conditions produced a suspension of black particles. An...

The Constitution of Status

Study of Melanin Bleaching After Immunohistochemistry of Melanin-containing Tissues

Melanin may interfere with immunohistochemical staining. The goal of this study was to investigate the effects of trichloroisocyanuric acid (TCCA) bleaching, potassium permanganate bleaching, and potassium dichromate bleaching on melanin, tissue antigen, and 3,3'-diaminobenzidine (DAB) using melanin-containing and melanin-free tissue samples. Our results demonstrated that all 3 bleaching method...

Isolation, Characterization of Melanin Producing Organism and Extraction of Melanin

Melanin producing organism was isolated from a soil sample of Ahmednagar district, Maharashtra using Tyrosine casein media. The isolated organism’s morphology and spore arrangement was studied. The isolated organism was suspected to belong to the genus Streptomyces. Parameter optimization of the Tyrosine casein media with respect to carbon and nitrogen source was done. Starch and L-glycine were...

Isolation and Characterization of Melanin Producing Pseudomonas stutzeri Strain UIS2 in the Presence of l-tyrosine and Survey of Biological Properties of Its Melanin

Background: Melanin is a negative charge hydrophobic complex pigment. Melanin is produced naturally in bacteria to protect them against UV, free radicals and environmental stresses. Pigment production in bacteria has more advantages than other biosources due to its rapid growth, higher efficiency and easier extraction. The aim of this study was the isolation, biochemical and molecular identific...